Testosterone Levels by Age in Men Age 20-44

Costa Rica Doctors Prescribe Testosterone Injections, Creams and Pellets for Men
Testosterone Levels by Age Chart
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Age Group | Mean morning T Level | 66th percentile | 75th percentile | 90th percentile | Upper physiological range |
20-24 | 501 | 558 | 604 | 757 | |
25-29 | 514 | 575 | 643 | 771 | |
30-34 | 456 | 498 | 559 | 678 | |
35-39 | 438 | 478 | 533 | 632 | |
40-44 | 430 | 473 | 504 | 640 |
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TRIIM Protocol includes HGH Injections

Jeff Ugalde, M.D.
Medical Director
We prescribe the same FDA-approved HGH injection pen used for short-stature in children. Adults and children use the same medication to increase growth hormone levels.
TRIIM HGH Anti-Age Protocol Summary
The TRIIM HGH Age Reversal Study used the following cocktail of medicines and dietary supplements:
- Human Growth Hormone (HGH)
- DHEA
- Vitamin C – 3,000 IU
- Zinc – 50 mg
Conclusion: After 1 year, participants showed reversal of biological age of 2.5 years, as measured by the Horvath Clock using DNA methylation aging tests.
Download: TRIIM 2019 Research Study.
Download: An epigenetic biomarker of aging for lifespan and healthspan.
Optimal Dosage of HGH for Anti-Aging
Most candidates prescribed Human Growth Hormone for Anti-Aging purposes based on the TRIIM Protocol will inject between 1 and 2 IU of HGH 3-5 days per week.
The optimal dose of HGH for adults undergoing an anti-age protocol will usually range between 1-2 IUs. These levels may be adjusted based on an individual basis, as adapted by observational blood tests.
What is the TRIIM Protocol?
The TRIIM Protocol is described in the 2019 study conducted by Dr. Gregory M. Fahy. See the video of his TEDx talk explaining the anti-age intervention study.
The basis of the protocol is to use Human Growth Hormone (HGH), Metformin, DHEA, and Vitamin D in order to rejuvenate the human Thymus, the major gland of the immune system responsible for manufacturing t-cells.
The TRIIM Protocol has been shown to reverse biological age as measured by the Horvath Clock used to calculate biological aging. Note that biological age is a more accurate measurement than chronological age. The numerical age of a person measured in years is less accurate at predicting longevity and health span than biological age.

Download a pdf of the Dr. Fahy Age Reversal TRIIM study.
HGH & Thymus
HGH is an abbreviation for human growth hormone. The growth hormone is a regenerative hormone that promotes the growth and regeneration of cells. The Thymus is the master gland of your immune system. It is located directly beneath your breastbone. It manufactures t-cells. T-cells help protect the body against illnesses including viruses and cancers.
As we age, the thymus begins to gradually degrade. Active healthy thymus tissue begins to diminish and get replaced by useless (unhealthy) fat. Hence, t-cells diminish and immunity is weakened.
From age 62 to 72, it is estimated that you lose approximately 98% of your capacity to fight off disease as measured by thymus function.
The TRIIM Protocol was designed by Dr. Fahy to study 9 adults for thymus regeneration using human growth hormone, metformin, DHEA, vitamin D, and zinc. The beneficial effects of growth hormone on aging cell regeneration have been documented since the 1990s, but Dr. Fahy is the first to publish a specific cocktail of medicines and dietary supplements to enhance the HGH replacement therapy.
HGH & Metformin
Metformin is a widely used medication for diabetes. Metformin helps to reduce insulin resistance. Additionally, it has long been considered an anti-aging medicine and is being studied for its longevity effects on its own.
The advantage of metformin in combination with HGH is it is believed to attenuate the insulin effect of growth hormone supplementation. Since growth hormone increases the body’s insulin-like growth factor levels, metformin is believed to offer some benefits in combination with growth hormone therapy in adults.
Because metformin may offer its own anti-aging benefits and has been prescribed safely for decades, it is well suited as an addition to standalone HGH therapy.
Adult Growth Hormone Age Limit
There is no growth hormone age limit for adults. Age Metrics Medical uses blood biomarkers obtained through periodic blood tests to determine if treatment is warranted. Assuming no pre-existing medical conditions that prohibit treatment with growth hormone, treatment can be prescribed no matter the age of the patient.
Does HGH Increase Cancer Risk?
For many years some have argued that adults taking human growth hormone may be at higher risk for developing cancer. However, the TRIIM study found that HGH therapy, combined with metformin, DHEA, and vitamin D may actually decrease the risk of cancer by reducing the biomarker PD1, which is a measurement of cancer risk. By increasing thymus function, risk factors for cancer may actually decrease in adults following the TRIIM protocol.
The TRIIM study also showed an increase in LMR. LMR is another biomarker linked to certain cancers. By increasing one’s LMR, it has been shown to improve outcomes for some with certain cancers.
Growth Hormone Treatment Cost - Costa Rica
Growth hormone therapy in Costa Rica is approximately 1/3 of the cost compared with the USA. We provide the same medication, by the same manufacturers, but for much less than in the United States.
A 10mg / 30 IU Norditropin HGH Pen in Costa Rica is roughly $550 USD.
Epigenetic Biological Aging Vs. Chronological Age
Epigenetic biological age is a more accurate measure of human age than the number of years one has lived. For example, we’ve all observed individuals who appear much younger than they actually are. This may be due to the fact that the epigenetic age (the biological age of their cells) is actually younger than others with the same chronological age. Biomarkers can now be used by doing a simple blood test and examining the results. Therefore, doctors and scientists can measure aging and biological age reversal in adults undergoing anti-aging protocols like those studied by Dr. Fahy in the TRIIM study.
Epigenetic clocks surpass chronological age in accuracy for estimating actual biological age.
Download: An epigenetic biomarker of aging for lifespan and healthspan.
Which HGH is the Best?
All FDA-approved human growth hormone prescriptions are molecularly identical. The main differences which may or may not be factors for some are delivery systems. We’ve found, after prescribing HGH for ten or more years, that Norditropin HGH is the preferred choice among our patients. Norditropin comes premixed in an easy-to-use injection pen. It can also go unrefrigerated for up to 14 days – making it very convenient for travel.

Best Time to Take HGH
Most doctors prescribe growth hormone injections right before bedtime. This is done to mimic the normal growth hormone cycle of the body. However, there is no definitive evidence that specifically states night is the only appropriate time to inject. It’s recommended that patients get periodic follow-up blood tests to evaluate the increase in growth hormone. This enables doctors to adjust the prescription to the optimal dosage for the patient.
How to Get HGH Treatment in Costa Rica
If you have an existing prescription for HGH, you can send or bring copies of your medical records to our clinic to refill your prescription. We can match or adjust the dosage and help to monitor treatment.
If you do not have an existing prescription, we can do preliminary blood testing and prescribe treatment on the same day of your visit. Most blood draws done at our clinic by 9 AM are back by the afternoon around 3-3:30 pm.
Map With Costa Rica Airport to Age Metrics

Conveniently located to San Jose’s Juan Santamaria Airport (SJO) in Escazú, San Jose COSTA RICA.
FAQ: TRIIM Study Anti-Aging Prescriptions
Approximately $550 monthly. This is for Norditropin HGH. Metformin, DHEA, Vitamin D and Zinc usually will add a small incremental fee for treatment.
The TRIIM Study was FDA approved as a clinical trial. However, it is not a standard treatment. The TRIIM protocol remains under investigation. The benefit of coming to Costa Rica is it is much easier to get a TRIIM Anti-Aging protocol without applying for enrollment into a research study. Note that the Human Growth Hormone Medication (Norditropin) is FDA approved in the United States.
Yes. Once prescribed your HGH becomes your personal use medication. You won't have trouble travelling with your treatment. We treat 100s if not 1,000s of medical tourists from the US and Canada annually.
Download the Norditropin Travel Document: [Norditropin Doctor Approved Travel Document].
No. Age Metrics Medical is not associated with the Life Extension Organization. However, our views on anti-aging are similar. For more information on Life Extension and the TRIIM Protocol, please visit the Life Extension Website.
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