Anti-Aging Triim Protocol, Reverse Biological Age

TRIIM Protocol Reverse Age
Reverse Biological Age
Costa Rica Doctors Prescribe TRIIM - Growth Hormone for Less

Anti-Aging TRIIM Protocol

The TRIIM Anti-Aging Trial examined nine healthy adult males between the ages of 51 and 65.

The men on average experienced 1.5 years of age reversal from baseline after 1 year of treatment (a −2.5‐year overall age reduction compared to no treatment at all).

A combination of nutrients, growth hormone, and a diabetic medication results in human age reversal, as measured by DNA methylation.

Our doctors can legally prescribe the TRIIM Protocol for anti-aging in Costa Rica. Medical tourism patients from the USA save 70% or more.

Countless medical tourists have come to us for growth hormone therapy for the last +9 years. 

Save 60-70% in Costa Rica on peer-reviewed HGH protocols shown to reverse aging based on objective scientific biological age markers. By using accurate age-related biomarkers, anti-age protocols are no longer inaccurately labeled as a medical hoax. Evidence-based “biological” anti-aging can show age reversal when prescribed and managed by an experienced medical team.

Age Metrics Medical COSTA RICA has been prescribing HGH-based protocols like the TRIIM study since 2013. We’ve treated thousands of patients with HGH safely and with positive results. 

Take a short flight from Atlanta, Miami, Dallas, Houston, and Los Angeles to San Jose Costa Rica, and get your TRIIM Study protocol. Fast, easy, and convenient with thorough testing and medical supervision. 

Where the Thymus Gland is Located and How it Impacts Aging

The thymus is a gland that is located in the chest, behind the sternum. It is an important part of the immune system, and it plays a crucial role in the development of T cells, which are a type of white blood cell that helps the body fight off infections. The thymus produces hormones that help to mature and activate T cells, and it also provides a supportive environment for T cells to develop and grow.

As people age, the thymus begins to shrink and produce fewer hormones, which can lead to a weakened immune system. This can make older people more susceptible to infections and other diseases. The decline in thymus function is a natural part of the aging process, and it is thought to be one of the reasons why older people are more susceptible to illness. However, there are also other factors that can contribute to the decline in thymus function, such as chronic diseases and poor lifestyle habits. It is important for people to take care of their health as they age to help maintain the function of the thymus and support a strong immune system.

The TRIIM protocol has been shown to help the thymus regenerate, grow larger, and result in the reversal of biological age.

TRIIM Protocol includes HGH Injections

Dr Jeff Ugalde Anti-Aging Costa Rica at Momentum

Jeff Ugalde, M.D.

Medical Director

We prescribe the same FDA-approved HGH injection pen used for short-stature in children.  Adults and children use the same medication to increase growth hormone levels. 

TRIIM HGH Anti-Age Protocol Summary

The TRIIM HGH Age Reversal Study used the following cocktail of medicines and dietary supplements:

  • Human Growth Hormone (HGH)
  • DHEA
  • Vitamin C – 3,000 IU
  • Zinc – 50 mg

Conclusion: After 1 year, participants showed a reversal of biological age of 2.5 years, as measured by the Horvath Clock using DNA methylation aging tests.

Download: TRIIM 2019 Research Study.

Download: TRIM 2019 Research Study Reversing Biological Age.

Download: An epigenetic biomarker of aging for lifespan and healthspan

The TRIIM Protocol in the News

Optimal Dosage of HGH for Anti-Aging

Most candidates prescribed Human Growth Hormone for Anti-Aging purposes based on the TRIIM Protocol will inject between 1 and 2 IU of HGH 3-5 days per week. 

The optimal dose of HGH for adults undergoing an anti-age protocol will usually range between 1-2 IUs. These levels may be adjusted based on an individual basis, as adapted by observational blood tests.

What is the TRIIM Protocol?

The TRIIM Protocol is described in the 2019 study conducted by Dr. Gregory M. Fahy. See the video of his TEDx talk explaining the anti-age intervention study. 

The basis of the protocol is to use Human Growth Hormone (HGH), Metformin, DHEA, and Vitamin D in order to rejuvenate the human Thymus, the major gland of the immune system responsible for manufacturing t-cells.

The TRIIM Protocol has been shown to reverse biological age as measured by the Horvath Clock used to calculate biological aging. Note that biological age is a more accurate measurement than chronological age. The numerical age of a person measured in years is less accurate at predicting longevity and health span than biological age. 

Download a pdf of the Dr. Fahy Age Reversal TRIIM study

HGH & Thymus

HGH is an abbreviation for human growth hormone. The growth hormone is a regenerative hormone that promotes the growth and regeneration of cells. The Thymus is the master gland of your immune system. It is located directly beneath your breastbone. It manufactures t-cells. T-cells help protect the body against illnesses including viruses and cancers. 

As we age, the thymus begins to gradually degrade. Active healthy thymus tissue begins to diminish and get replaced by useless (unhealthy) fat.  Hence, t-cells diminish and immunity is weakened.

From age 62 to 72, it is estimated that you lose approximately 98% of your capacity to fight off disease as measured by thymus function.  

The TRIIM Protocol was designed by Dr. Fahy to study 9 adults for thymus regeneration using human growth hormone, metformin, DHEA, vitamin D, and zinc. The beneficial effects of growth hormone on aging cell regeneration have been documented since the 1990s, but Dr. Fahy is the first to publish a specific cocktail of medicines and dietary supplements to enhance the HGH replacement therapy.   

HGH & Metformin

Metformin is a widely used medication for diabetes. Metformin helps to reduce insulin resistance. Additionally, it has long been considered an anti-aging medicine and is being studied for its longevity effects on its own.

The advantage of metformin in combination with HGH is it is believed to attenuate the insulin effect of growth hormone supplementation. Since growth hormone increases the body’s insulin-like growth factor levels, metformin is believed to offer some benefits in combination with growth hormone therapy in adults. 

Because metformin may offer its own anti-aging benefits and has been prescribed safely for decades, it is well suited as an addition to standalone HGH therapy. 

What is Human Growth Hormone?

Human growth hormone (HGH) is a hormone produced by the pituitary gland in the brain. It is involved in many important processes in the body, including growth and development, metabolism, and the breakdown of fats and sugars. HGH is essential for normal growth and development during childhood and adolescence, and it continues to play a role in various physiological processes throughout life.

HGH is produced in pulses throughout the day, with the highest levels occurring during childhood and adolescence and declining with age. The production of HGH is regulated by a number of factors, including age, diet, exercise, and sleep.

In some cases, HGH deficiency can lead to short stature and other health problems. HGH therapy, which involves the use of synthetic HGH or HGH releasing agents, may be used to treat certain conditions, such as HGH deficiency or Turner syndrome (a genetic disorder that affects growth and development in girls).

What is Metformin Used For?

Metformin is a medication that is primarily used to treat type 2 diabetes. It belongs to a class of drugs called biguanides, which work by decreasing the amount of sugar (glucose) produced by the liver and increasing the sensitivity of muscle cells to insulin, a hormone that helps regulate the level of glucose in the blood.

Metformin is usually taken orally in the form of a pill or liquid. It is usually taken once or twice a day, with or without food. The dosage of metformin is usually started at a low level and gradually increased to minimize side effects, which can include stomach upset, diarrhea, and bloating.

In addition to its use in the treatment of type 2 diabetes, metformin has also been studied for its potential use in the treatment of other conditions, including obesity, polycystic ovary syndrome (PCOS), and nonalcoholic fatty liver disease (NAFLD). However, the safety and effectiveness of metformin for these conditions have not been fully established, and further research is needed.

Adult Growth Hormone Age Limit

There is no growth hormone age limit for adults. Age Metrics Medical uses blood biomarkers obtained through periodic blood tests to determine if treatment is warranted. Assuming no pre-existing medical conditions that prohibit treatment with growth hormone, treatment can be prescribed no matter the age of the patient. 

Does HGH Increase Cancer Risk?

For many years some have argued that adults taking human growth hormone may be at higher risk for developing cancer. However, the TRIIM study found that HGH therapy, combined with metformin, DHEA, and vitamin D may actually decrease the risk of cancer by reducing the biomarker PD1, which is a measurement of cancer risk. By increasing thymus function, risk factors for cancer may actually decrease in adults following the TRIIM protocol. 

The TRIIM study also showed an increase in LMR. LMR is another biomarker linked to certain cancers. By increasing one’s LMR, it has been shown to improve outcomes for some with certain cancers. 

Growth Hormone Treatment Cost - Costa Rica

Growth hormone therapy in Costa Rica is approximately 1/3 of the cost compared with the USA. We provide the same medication, by the same manufacturers, but for much less than in the United States. 

A 10mg / 30 IU Norditropin HGH Pen in Costa Rica is roughly $550 USD. 

Epigenetic Biological Aging Vs. Chronological Age

Epigenetic biological age is a more accurate measure of human age than the number of years one has lived.  For example, we’ve all observed individuals who appear much younger than they actually are. This may be due to the fact that the epigenetic age (the biological age of their cells) is actually younger than others with the same chronological age. Biomarkers can now be used by doing a simple blood test and examining the results. Therefore, doctors and scientists can measure aging and biological age reversal in adults undergoing anti-aging protocols like those studied by Dr. Fahy in the TRIIM study. 

Epigenetic clocks surpass chronological age in accuracy for estimating actual biological age.

Download: An epigenetic biomarker of aging for lifespan and healthspan

Which HGH is the Best?

All FDA-approved human growth hormone prescriptions are molecularly identical. The main differences which may or may not be factors for some are delivery systems. We’ve found, after prescribing HGH for ten or more years, that Norditropin HGH is the preferred choice among our patients. Norditropin comes premixed in an easy-to-use injection pen. It can also go unrefrigerated for up to 14 days – making it very convenient for travel. 

Costa Rica HGH Pen

Best Time to Take HGH

Most doctors prescribe growth hormone injections right before bedtime. This is done to mimic the normal growth hormone cycle of the body. However, there is no definitive evidence that specifically states night is the only appropriate time to inject. It’s recommended that patients get periodic follow-up blood tests to evaluate the increase in growth hormone. This enables doctors to adjust the prescription to the optimal dosage for the patient.

Where can I get the TRIIM Anti-Aging Protocol?

The TRIIM Protocol, although relatively new by HGH therapy standards, has been prescribed by our doctors in Costa Rica since 2013. You can receive Human Growth Hormone in the Novonordisk NordiFlex Pre-Mixed Injection Pen and other supplements prescribed by our doctors on the same day.  We perform qualifying blood tests in the morning and prescribe the treatment plan in the afternoon, once the doctor has time to review your lab results. 

Get the TRIIM Anti-Aging protocol prescribed by a board-certified anti-aging doctor in Costa Rica. 

 Before it was known as the TIIM protocol, it was a series of HGH and other supplements created to provide anti-aging benefits such as:

  • Improved body composition
  • Increase in muscle mass
  • Decrease in body fat
  • Increased skin elasticity and thickness
  • Improved mental acuity
  • Improved memory and a sense of well-being

If you have an existing prescription for HGH, you can send or bring copies of your medical records to our clinic to refill your prescription. We can match or adjust the dosage and help to monitor treatment. 

If you do not have an existing prescription, we can do preliminary blood testing and prescribe treatment on the same day of your visit. Most blood draws done at our clinic by 9 AM are back by the afternoon around 3-3:30 pm. 

How does Vitamin D Help the Body?

Vitamin D is a fat-soluble vitamin that is essential for maintaining healthy bones and teeth. It is also important for supporting the immune system and helping the body absorb calcium, a mineral that is necessary for strong bones.

There are several ways in which vitamin D helps the body:

  • Maintaining bone health: Vitamin D helps the body absorb calcium and phosphorous, which are necessary for the formation and maintenance of healthy bones and teeth.
  • Supporting the immune system: Vitamin D helps to activate immune cells, such as T cells and macrophages, which help to protect the body against infections and diseases.
  • Regulating blood pressure: Vitamin D may help to regulate blood pressure and reduce the risk of high blood pressure.
  • Reducing inflammation: Vitamin D has anti-inflammatory properties and may help to reduce inflammation in the body.
  • Reducing the risk of certain diseases: Some studies have suggested that vitamin D may help to reduce the risk of certain diseases, such as osteoporosis, diabetes, and certain types of cancer.

Vitamin D can be obtained from certain foods, such as fatty fish, egg yolks, and fortified foods, and it can also be synthesized by the body when the skin is exposed to sunlight. However, many people do not get enough vitamin D from their diet or from sun exposure and may need to take supplements to meet their daily requirements.

How does Zinc Help the Body?

Zinc is a mineral that is essential for the proper functioning of the body. It is involved in a wide range of biological processes, including immune function, wound healing, taste, and smell.

Here are some ways in which zinc helps the body:

  • Immune function: Zinc plays a crucial role in the immune system, helping to activate immune cells and produce antibodies. It may also help to reduce the severity and duration of certain infections.
  • Wound healing: Zinc is necessary for the production of collagen, a protein that is essential for wound healing.
  • Taste and smell: Zinc is involved in the sense of taste and smell, and a deficiency in zinc can affect these senses.
  • Growth and development: Zinc is necessary for normal growth and development during pregnancy, childhood, and adolescence.
  • Protein synthesis: Zinc is required for the synthesis of proteins, which are the building blocks of the body.
  • DNA synthesis: Zinc is necessary for the synthesis of DNA, the genetic material that carries the instructions for the development and function of living organisms.

Zinc can be found in a variety of foods, including meat, seafood, nuts, seeds, and whole grains. It is also available in dietary supplements.

Map With Costa Rica Airport to Age Metrics


Conveniently located to San Jose’s Juan Santamaria Airport (SJO) in Escazú, San Jose COSTA RICA.

FAQ: TRIIM Study Anti-Aging Prescriptions

Approximately $550 monthly. This is for Norditropin HGH. Metformin, DHEA, Vitamin D and Zinc usually will add a small incremental fee for treatment.

The TRIIM Study was FDA approved as a clinical trial. However, it is not a standard treatment. The TRIIM protocol remains under investigation. The benefit of coming to Costa Rica is it is much easier to get a TRIIM Anti-Aging protocol without applying for enrollment into a research study. Note that the Human Growth Hormone Medication (Norditropin) is FDA approved in the United States.

Yes. Once prescribed your HGH becomes your personal use medication. You won't have trouble travelling with your treatment. We treat 100s if not 1,000s of medical tourists from the US and Canada annually. 

Download the Norditropin Travel Document: [Norditropin Doctor Approved Travel Document].

No. Age Metrics Medical is not associated with the Life Extension Organization. However, our views on anti-aging are similar. For more information on Life Extension and the TRIIM Protocol, please visit the Life Extension Website.

Age Management & Aesthetics

Various medical weight-loss options. HCG, GLP-1 mimetics, and other alternatives. 

HGH, DHEA, Metformin prescribed based on the 2019 TRIIM Study.

Bioidentical Hormone Replacement Therapy Overview.

Hormone Replacement

Bioidentical hormone replacement therapies for men and women. 

Order your bioidentical hormone therapy blood testing in the USA.

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PRP for Joint Repair

Platelet-rich Plasma injections for joint repair. 

P-SHOT PRP Injections

Penile Platelet-rich Plasma cell-based injections for ED. Other ED therapies. 

Micro-needling and Platelet-rich Plasma for entire face rejuvenation. 

Platelet-rich plasma and micro-needling for hair follicle rejuvenation. 

Brand name BOTOX and Fillers. BOTOX, Dysport, and approved facial fillers. 

IV Therapies

Myers’ cocktail, high-dose vitamin C, Chelation, and other common nutrient IV therapies.

Bioidentical hormone replacement therapies for men and women. 


Nutrition coaching for improved energy metabolism and fat loss. Keto & plant-based options. 

Bio-identical hormone replacement with pellet implants. Women and men can benefit.

Myers’ cocktail, high-dose vitamin C, Chelation, and other common nutrient IV therapies.

Save thousands on Growth Hormone Therapy for idiopathic short stature (ISS) in children.


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